Using CRISPR-CAS9 technology to publish high score articles

The title party Dr.S is coming again, but every time the article can receive positive feedback from the readers, Dr.S is still very pleased, so we will continue to provide you with a popular idea of ​​easily publishing high-scoring SCI papers.

We use the gene chip, genome-wide association analysis, second-generation sequencing and other technologies to conduct high-throughput-related gene screening to publish high-scoring articles. We are still in the eye, and a new revolution is coming. The only strains that may be related to the screening by the previous methods are not known. Some of the research groups spent the old time on sequencing and got a bunch of so-called key genes, which is impossible to start with. Now that I have entered the age of functional genetic research, how can genes make people reviewers and editors happy? Don't be afraid, isn't this new technology for functional gene screening coming? The following two pictures are believed to be familiar to you. It is the susceptibility of vemurafenib (PLX) drugs in A375 cells using the CRISPR-CAS9 and its improved technology published by Dr. Zhang Feng in the top magazine for two consecutive years. / Drug resistance gene screening work.

Almost exactly the same idea, but the method changed from CAS9 Loss-of-Function (LOF) screening to SAM Gain-of-Function (GOF) screening.   Please click here for a detailed explanation of the two articles.   ,   Please click here for a technical introduction to SAM . Is it a little bit of watering? Don't worry, articles of the same idea are being published. Let's take a look at which areas can be easily published using CAS9 or SAM technology for high-throughput screening of functional genes. The following two articles are dedicated to genes that regulate anthrax and West Nile virus infection.

The idea is exactly the same as Zhang Feng's two articles. About 77,000 sgRNA viruses are constructed to form a virus library. The virus library can infect the library with a large number of cells for the 20121 human gene, and adjust the infection volume to ensure most of the infection. Only one gene in the cell was knocked out. These deadly anthrax or West Nile virus are then used to infect these knockout cells, and the surviving cells are deeply sequenced to obtain enriched sgRNAs. It is precisely because these sgRNAs successfully knock out the target genes, resulting in even cells. If a virus that is infected with a deadly virus can survive, then these genes may become effective drug targets for the treatment of these viral infections.

How about, after reading these articles, can we also find out what is keen? Yes, it’s a good time to catch up with Nature Genetics without catching up with GWAS. Whether it is to study key genes for tumor proliferation, drug resistance genes, viral infections, or other fields, as long as the research direction is about cell birth or death, you can use this method! There are so many cancers, so many drugs, so many pathogenic viruses, choose a cell line, a drug or a virus to screen for a high-throughput functional gene, and the resulting genes can be studied in depth, and the seed genes obtained. It may be used for subsequent 5-10 years of research. And others who have done CAS9 LOF research can also use SAM GOF to re-screen once, what are they waiting for, and then they will be sent out by others!

Jikai Gene can provide you with high-quality CAS9 and SAM target virus database to help you quickly perform functional gene screening. I wish you a high-scoring SCI paper soon !

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