1. The collected heart and lung tissue samples are placed into a 5ml crusher grinding tube ( or a common round bottom centrifuge tube ) , and each tube is placed in an amount of about 1/3 of the volume of the single tube; 2. Insert 3 stainless steel grinding beads with a diameter of 8mm in each centrifuge tube, cover the tube cover, insert the centrifuge tube into the adapter symmetrically, and fix the upper rocker arm; 3. Set the TL2010S tissue breaker parameter to 1800 rpm , automatically stop grinding 10S and cycle 5 times per grinding for 60 seconds (ie intermittent grinding for 5 minutes); After the grinding process is completely stopped, the centrifuge tube is taken out and opened. The state of the sample after the crushing is as shown in the photo of Figure 2, and the sample is broken very uniformly: Figure 1 : TL2010S Tissue Breaker Real Machine Photo Figure 2 : On-site real shot of broken heart and lung tissue ( using a normal 5ml centrifuge tube ) Fruit juice drinks is a beverage made of fruit juice products obtained by physical methods such as pressing, centrifugation and extraction. Fruit juice retains most of the nutrients in the fruit, such as vitamins, minerals, sugar and pectin in dietary fiber. Often drinking fruit juice can help digestion, moisten the intestines and supplement the lack of nutrients in the diet. Juice Drinks,Honey Tea,Honey Citron Tea,Goji Berry Juice Xi'an Gawen Biotechnology Co., Ltd , https://www.seoagolyn.com
Why can't laboratory personnel use a conventional tissue homogenizer before to achieve better crushing?
Through a large number of sample crushing tests, Ding Yiyuan Technology found that animal tissue samples are more difficult to break than other samples, especially some connective tissue, heart tissue, and lung tissue. Because of the high toughness, it is generally difficult to use ordinary manual grinding or The conventional tissue homogenizer is completely broken, so it cannot meet the subsequent detailed experimental requirements. With the TL2010S tissue breaker , high-speed crushing of multiple samples can be carried out for different volumes and quantities of samples, with different sizes of centrifuge tubes and adapters. Not only is the operation flexible and convenient, but also avoids cross-contamination between samples, eliminating the tedious task of cleaning the instrument.
Why use a 5ml breaker grinding tube ( or a normal round bottom centrifuge tube ) ?
We know that the TL2010S tissue breaker can also be milled with multiple 1.5/2.0ml centrifuge tubes, but in this experiment , the centrifuge tube of this size is smaller than the coarse sample to be crushed in large quantities, and it can be accommodated. The grinding beads are also small, so the impact force is limited and the best grinding effect is not achieved. With a 5ml crusher grinding tube ( or a normal round bottom centrifuge tube ) , the amount of sample placed in a single tube is not only increased, but a larger size of the grinding beads can be placed to create a larger impact force for better grinding. effect.
Why set up intermittent grinding and breaking?
Intermittent grinding is one of the features of the TL2010S tissue breaker . Setting a certain interval time can effectively reduce the degradation of the sample due to rapid temperature rise during continuous high-speed grinding and fracture, and provide guarantee for the smooth progress of subsequent experiments. If combined with liquid nitrogen treatment, it can reduce the temperature rise of the sample more effectively, which is good for temperature-sensitive experiments such as RNA extraction.
Finally, a good method for quickly and efficiently breaking heart and lung tissue is summarized into the following formula for your reference:
Good method for quickly and efficiently breaking heart and lung tissue = TL2010S tissue disrupter + 5ml centrifuge tube and adapter + 3 tubes of 8mm diameter 8mm grinding balls + 5 minutes intermittent grinding ( 10 seconds every 60 seconds, 5 cycles)
There are two kinds of fruit juice: pure fruit juice and compound fruit juice. As far as pure fruit juice is concerned, it can be divided into two types: one is to directly use the original juice, and the other is to dehydrate the freshly squeezed fruit juice first, and then add water to reduce it into concentrated fruit juice. Most pure fruit juices are concentrated. AGOLYN offers various flavors of honey tea, such as honey Citron tea etc.
How to quickly and efficiently break animal heart and lung tissue samples with TL2010S tissue breaker
A laboratory at the Fourth Military Medical University recently solved an annoying problem. When they are doing drug metabolism analysis experiments, they need to crush a large number of collected rabbit heart muscle tissue and lung tissue samples for subsequent comparative analysis of different methods. However, using the existing tissue homogenizer in the laboratory, not only the treatment flux is small, easy to be contaminated and difficult to clean, the sample after treatment is not uniform and meticulous, and the requirements for subsequent analysis experiments are basically not met. For this reason, the experimental project was once unable to be carried out on a large scale. In November 2014, the laboratory staff contacted Dingyiyuan Technology to apply for the trial of TL2010S tissue breaker ( also known as TL2010S flux milling grinder ) for sample crushing. With the assistance of Dingyuanyuan technology engineers, they finally found out A good method for quickly and efficiently breaking up heart and lung tissue samples in batches has the following main operational points:
Next Article
How do men eat beef?
Prev Article
Egg production date feed use points