β-Tubulin Antibody - Make internal reference to beautiful WB and IHC images

Tubulin, a tubulin, is a backbone protein of cells. Tubulin is divided into various tubulins such as α, β, γ, δ, ε, etc. Among them, α-Tubulin and β-Tubulin can form heterodimers, which are the two main types of Tubulin which form microtubules. The molecular weights of α-tubulin and β-tubulin were 55kDa and 50kDa, respectively, and the actual detection bands were all around 55kDa. As an internal reference, the protein level of β-tubulin is usually not changed, so it is widely used as a reference for consistent sample loading in Western Blotting. It is also often used for immunostaining to observe the microtubule structure of cells.

How to choose the appropriate β-Tubulin antibody?

Although there are many choices of internal reference antibodies, they also need to follow certain principles and meet the practical application requirements. First, we need to consider the size of the protein of interest. We recommend that the internal reference should have a molecular weight difference of more than 5KD from the target protein. Therefore, you need to select the appropriate internal reference according to the molecular weight of the protein for your purpose, due to the molecular weight of β-Tubulin. At approximately 55 kD, the internal reference antibody is not well suited for use in WB assays for detecting 50 kD-60 kD size target proteins. In addition, there are certain differences in the selection of internal reference samples from different tissue sources. For example, in a small number of special cases, such as adipose tissue or intracellular, β-Actin is rarely expressed, and is not suitable for internal reference.

The first purchase of Abbkine's β-Tubulin antibody was used for cellular immunofluorescence. Later, the tissue block was used as a sample and colocalization was required. I tried to use this antibody to locate the cytoskeleton β-Tubulin and make immune tissue. Fluorescence, the effect is not bad, it is a bit unexpected. ——One customer of the Third Hospital of Peking University School of Medicine

In addition, we also need to consider our own needs, especially the needs of experimental applications. In general, the more types of applications, the greater the choice in use, and the monoclonal antibodies from mouse sources generally have better specificity, stability and potency than polyclonal antibodies. In addition, the titer of the antibody is also an important aspect to examine the cost performance of the antibody, which is equivalent to the dilution ratio in practical use. A high titer of 100 μl β-Tubulin antibody (if the dilution ratio of WB is 1:5000, the final solution is equivalent to 500 ml), 1 ml of β-Tubulin antibody with lower potency (if the dilution ratio of WB detection) It is 1:200, and the final use solution is equivalent to 2ml). Therefore, when selecting antibodies, not only the amount of antibody needs to be considered, but also the titer of the antibody, that is, the recommended dilution ratio for different applications.

Why choose the Abbkine beta-Tubulin antibody?

There are many brands that supply β-Tubulin antibodies, such as Abcam, Santa Cruz, Cell Signaling, Sigma, and even Millipore, R&D, Novus, BD, etc. Here is a simple comparison of several imported brands:

Brand	Item number	2013 offer	specification	App types
	Ab6046	¥4418	100μg/100μl	ICC/IF, IHC, WB
	2146S	¥2997	100μl	WB, IF, ELISA
	T4026	¥4140	200μl	WB, IF
	A01030	¥1800	200μl	WB, IHC

In addition, some domestic suppliers can also provide beta-Tubulin antibodies, but there are deficiencies in brand and quality assurance. The domestically packaged β-Tubulin antibody is insufficient in quality stability, and its valence is also lost after being packaged. Some β-Tubulin antibodies originating in domestic laboratories have better detection activity, but there are gaps in application types, and it is difficult to control the stability between batches, while only a small amount of origin is produced in China. Antibodies are also polyclonal antibodies. If you want to buy a beta-Tubulin internal reference antibody that is more cost-effective and also an imported brand, then Abbkine may be one of your important considerations. We recommend Abbkine's Beta-Tubulin Reference Anti-beta Tubulin Monoclonal Antibody (3G6) (Cat. No. A01030).

The antibody monoclonal antibody has the clone number 3G6. Its antigen is a specific and conserved sequence in β-Tubulin. One advantage is that it is widely detected in mice, rabbits, rats, monkeys, dogs, cats, guinea pigs, etc. Widely recognizable, in particular, the antibody is also suitable for zebrafish samples, and is also a truly conserved internal reference antibody. In addition, the titer of this antibody is very high, the recommended dilution ratio for WB experiments is 1:1000-1:10,000, and even customers have reported higher dilution ratios.

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The anti-fluorescence effect of the β-Tubulin internal reference antibody is also excellent, and is an antibody product which is very suitable for immunohistochemical staining of the cytoskeleton. In addition, some customers reported that the antibody was used for fluorescent staining of tissues, and unexpected effects were also obtained.

In addition to the 100μl small package, Abbkine also offers a large package of 500ug of β-Tubulin internal reference antibody, which is ideal for routine internal validation or calibration, or for immunological experiments in cytoskeletal fluorescence localization. For the convenience of customers, Abbkine is available in stock in China. Abbkine provides global product quality tracking and after-sales service and protection for the Chinese market. If you have any problems during the use, please contact your local agent Ai Meijie Technology Co., Ltd. directly.

The American Abbkine brand is a brand that provides cost-effective immunology and cytology research products and solutions. Amy Jie introduced it to China as its regional agent in China, and will provide customers with the most cost-effective routine immunological reagents, such as A tag antibody, an internal reference antibody, a conventional primary antibody, and a full range of secondary antibodies, antibody detection substrates, and related kits. At the same time, as a Chinese agent or regional agent of many international famous brands, Aimei can provide you with a series of laboratory solutions. If you are interested in the above products, please call the free customer line 400-6800-868 to 801/802/803 or 027-84454412/4876 to contact Aimei Technology Co., Ltd. for product information and complete experimental solutions.

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