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Discussion on the advantages and disadvantages of spectrophotometry and measurement in pipette calibration (2)
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What is spectrophotometric calibration?
Let's first understand what is spectrophotometry, please see the schematic below: (The following picture is omitted)
The principle of spectrophotometry calibration is to mix a liquid ____ solvent (English solution) with a special substance, pipette it into the target container, and then filter out a part of the unwanted wavelength light by using a specific filter. Then, the remaining specific wavelength of light is measured, thereby calculating the liquid actually sucked and discharged by the pipette. Throughout the principle of spectrophotometric calibration, the factors that may affect the calibration results during the calibration of the pipette by spectrophotometry are:
1 temperature, because spectrophotometry also requires the use of liquids, ie solvents, a certain amount of solvent, the volume of which is also affected by temperature;
However, if the pipette is calibrated at the user's location, this effect can be ignored, because the temperature change in the location of a particular user is relatively fixed, then this temperature is brought about. The effect on the volume in the spectrophotometric calibration will also exist in the actual work of the user, so the two can cancel each other out;
2 Air pressure, like the effect of temperature, the effects of air pressure can also cancel each other out;
3 The influence of the operator's operating habits, because spectrophotometry is a pipette calibration method that can be applied directly to the end user's place of use, and its operation method is simpler and more reliable than the traditional measurement method. The pipette can be directly used by the user of the pipette to calibrate the pipette used by the spectrophotometer. Therefore, the difference in the operator's operating habits during the calibration process also exists in the future use. Therefore, the difference between the two can also cancel each other out;
4 Ambient humidity, because spectrophotometry does not weigh the solvent discharged from the pipette, but measures the light wave of the special substance contained in the solvent. At the same time, this special substance is non-volatile, so the whole test In the process, the solvent that has been discharged through the pipette, regardless of its quality, is not affected, that is, it does not require control of the dry humidity of the environment.
Therefore, we can almost say that the pipette is calibrated with spectrophotometric emission, and the whole test process is almost independent of environmental factors, such as temperature, air pressure, dry humidity, etc., and because during this calibration process, The calibrator and the end user are the same person, so the difference caused by human factors is also minimized.
In the process of calibrating the pipette by spectrophotometry, the only uncompensable difference is the error of the spectrophotometric device and the reagent itself, which is the same as the error of the measuring instrument itself in the traditional measurement method, such as the electronic balance. The error of the own, the error of the thermometer itself, the error of the first-stage barometer of the dry hygrometer, etc., but these errors from the measuring device and the solvent itself are usually constant, and can be measured in advance, and the measurement data that can be passed Make corrections to avoid it.
Of course, the current spectrophotometry as a new application method for pipette calibration is still emerging. There are only a few research institutes in foreign countries that use it. In China, it is only at the theoretical level. It has not been promoted and applied in practice. However, because the spectrophotometry is not interfered by environmental factors, the application environment is more random and wide. Therefore, the author is artificial. This new method for pipette calibration will be more recognized by users in the near future. With recognition.
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