How to quickly understand a high score SCI article

Dear readers, thank you for your interest in the high-scoring SCI article analysis series before the Dr.S. There have been a lot of reader feedback in the near future. Is there a simpler and more rude way to quickly understand a high score SCI article? Dr.S wants to say why didn't you say it earlier? Of course there is. From today, Dr.S will open a new series dedicated to our high-TOU (LAN) readers.

If you want to send an article with a score of about 10 points, at least what conditions are required. Let's review the routines that Dr.S taught you before:

→ A new functional gene

→ Three levels of research on new genes (clinical relevance, functional verification, mechanism discussion)

We will refine these routines to form a test paper on the subject overview, fill in the empty space, and you can try to submit the manuscript after the project is selected. Is it very simple and rude?

1. The new gene was found by ___________.

2. The verified gene is in the ___________ functional direction.

3. In order to verify gene function, experimental methods such as _________________ were used.

4. The new gene is functioning through the ________________ pathway/key molecule.

5. Is the new gene clinically relevant? (Yes or no)

6. Does the new gene function at both in vitro and in vivo levels? (Yes or no)

7. Does the mechanism study do a functional recovery experiment for downstream key molecules? (Yes or no)

8. Has the mechanism study verified the direct interaction with downstream key molecules? (Yes or no)

Is it very simple, we can use a recently published Hepatology article Up-Regulation of Histone Methyltransferase SETDB1 by Multiple Mechanisms in Hepatocellular Carcinoma Promotes Cancer Metastasis to test our examination papers.

answer:

1. The new gene was screened by expression profiling to screen cancer and adjacent tissues of liver cancer patients .

2. The verified gene is in the direction of proliferation and metastasis .

3. In order to verify gene function, cell proliferation experiments, Transwell, and tumor formation in nude mice were used.

4. The new gene functions through regulation by Sp1 and miR-29 .

5. Is the new gene clinically relevant? Yes

6. Does the new gene function at both in vitro and in vivo levels? Yes

7. Does the mechanism study do a functional recovery experiment for downstream key molecules? no

Ps: This article did not do a reply experiment, but the article was also successfully received, but I suggest you add this part to make the logic more complete to block the questions that reviewers may ask.

8. Has the mechanism study verified the interaction with key upstream and downstream molecules? Yes (this article uses the fluorescein reporting system)

If you want to send high scores, each article must be filled, and the next four questions must be "Yes"!

According to the experience of the Jikai gene, the probability of high-throughput screening of new functional genes from the sample by chip, sequencing, and mass spectrometry is about 10%. It is very difficult to screen and verify a new functional gene. After three years of research and development, Jikai has completed nearly 6,000 functional-related screening of tumor-related genes, and has identified a number of clear tumor function markers, which can quickly match your high-throughput data and help you save functional genes. Screening costs , waiting for you to hunt for treasure ~~~

Special Intermediates

Our special intermediates are mainly a combination of our own actual product structure and product properties, including: 1. The product does not belong to any other product of our company intermediates, 2. Chemical reaction type is more special 3. Can be used to synthesize two or more kinds of API which roles is almost equivalent and difficult to separate the level. 4. This intermediate is somewhat less used for actual production or less practical use. We summarized the above four cases of intermediates temporarily as a special intermediate, if the special intermediate application is enlarge, we will adjust it according to the actual situation to change its "identity."

Special Intermediates,Tofisopam Intermediates,Amisulpride Intermediates,Antithrombotic/Blood thinners

Taizhou Volsen Chemical Co., Ltd. , https://www.volsenchem.com