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How to choose a microscope and camera
Microscopes can be divided into bright field microscopes, dark field microscopes, phase contrast microscopes, fluorescence microscopes, DIC microscopes, HMC microscopes, etc. The choice of microscope and microscope camera has a lot to do with the type and state of the sample. Here are some examples of how to choose a microscope and a microscope camera from the microscope sample.
Microscopic samples are divided into several types:
1. Stained samples (cell samples) and thicker samples (tissue sections)
Sample characteristics: The color is more vivid, and the microstructure to be observed has a larger color or color difference from the periphery.
Suitable microscope: bright field microscope (bright field) is also called bright field microscope, such as Olympus CX21, CX31, CX41; Nikon's E100, E200; lookout BL10, BL32; high-end Olympus BX series Wait.
Suitable digital camera: color CMOS or CCD camera, such as our company's MD50, MC50, MD90.
Reason: For color samples, color digital cameras can preserve beautiful colors, so color is a must. The bright field microscope makes the exposure time of the digital camera very short, so the noise is less demanding on the camera.
2. Contrast (unstained) samples, very small samples
Characteristics of the sample: The difference between the observed part and the surrounding part is small, the contrast is poor, or the sample is small and not easy to observe, such as Olympus, CX41 dark field, BX53 dark field, etc.
A suitable microscope: Dark field is also called dark field microscope.
Suitable digital camera: color CMOS or CCD camera, such as cost-effective MC15; 9 million pixel high definition MD90; large target CCD MC50 is a good choice.
3. Samples for microbial identification and morphological observation
Microbial identification is usually observed by living cells, and the understanding of the shape of the microorganisms is required, which is important for edge enhancement.
Suitable microscopes: phase contrast microscope (Phase Contrast), Olympus microscope CKX41, CKX31, IX53, IX73, and the like.
Suitable camera: MD50 of 5 million pixels; MD90 of 9 million pixels; MC50 with high sensitivity of 5 million pixels.
4. Ultra-thin section
Features: The highest transparency sample, the sample boundaries are not clear enough.
Suitable microscopes: Differential Interface Contrast, Olympus IX73, etc.
Suitable camera: 12bit black and white CCD, the camera's spatial resolution and gray level can get better results when the boundary is not clear, 1.4 million pixel black and white cold CCD MC21; 5 million pixel cold CCD MC50.
5. Crystals, polymers
Features: The sample itself has a polarizing reaction.
Suitable microscope: Polarization microscope, such as Olympus microscope BX51P, BX53P and so on.
Suitable digital camera: best use CCD; 1.4 million cost-effective MC15; 3 million pixel CCD--MC30.
6. Chloroplast, fluorescence microscope samples
Features: There is a fluorescence reaction, and attention should be paid to the wavelength of the excitation and emission light. Divided into inverted fluorescence and upright fluorescence, such as Olympus BX53, IX73; our LF51, LF52; the latest LED upright fluorescence microscope LF10-LED and LE30-LED.
Suitable microscope: Fluorescence.
Suitable digital camera: Cooling CCD, reducing noise output to ensure better image quality in low light conditions. Such as the 12.5 megapixel cold CCD MCX2; 5 megapixel cold CCD MC50 and MC55; 1.4 megapixel cold CCD MC20 and MC21 are good choices.
For more information on the product, please contact Mr. Zhou Mobile at 17705152197, 025-85657976.
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