Application of Namocell Single Cell Separator---Monoclon Screening and High-yield Cell Line Selection

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The production of monoclonal antibody technology has been relatively mature. Monoclonal antibodies have the characteristics of strong targeting, high specificity and low toxic and side effects, and have been successfully used in the treatment of various diseases such as immunity and cancer. Therapeutic monoclonal antibody drugs have become the most widely used type of biotechnology drugs and the largest sales. The proportion of globally marketed anti-drugs in biopharmaceuticals is over 30%, and the proportion of anti-monoclonal drugs in biopharmaceuticals is over 70%. Compared with the international level, the use rate of monoclonal antibody in China is still relatively low, but the compound annual growth rate of China's monoclonal antibody market in the past 10 years is close to 50%.

Monoclonal antibody is a research and development hotspot in the world. At present, more than 200 companies in the world are engaged in the research and development of antibody drugs, and tens of thousands of monoclonal antibodies have been produced. Even so, the current search for new monoclonal antibodies by researchers has never stopped. How to quickly, efficiently and accurately screen high-affinity monoclonals and how to select cell lines to increase yield has become a major concern for many pharmaceutical companies. With its unique new separation technology, Namocell offers a successful solution to both of these issues.

First, monoclonal antibody screening

The traditional method has a long cycle and a low efficiency when selecting a single clone. In addition, because hybridoma cells bring a lot of unnecessary problems, everyone hopes to avoid this link.

With Namocell's original enrichment sorting, very low levels of immune B cells can be selected, resulting in a much shorter overall process. By screening this process, high-affinity monoclonal B cells can be obtained without the need to fuse the tumor cells.

Second, high-yield cell line selection

The traditional limited dilution method : the operation is cumbersome, the time period is long, the monoclonal rate is low, and high-yield cells cannot be selected when plating.

Semi-solid medium selection method : The monoclonal rate is greatly improved. The downside is that there are differences in the growth environment and the production of antibodies by cells. High-yield cells selected on semi-solid media do not necessarily have high yields in a large-scale suspension environment.

Flow cytometry selection method : Sorting speed is fast. The disadvantage is that the sheath fluid pressure is relatively large, the damage to the cells is relatively heavy, and the cell growth rate is low.

Namocell selection method : Microfluidic technology enables cell sorting in a gentle low pressure environment (below 2 psi). Using laser excitation and fluorescence detection detection principles, high-yield and high-activity cells can be selected. Efficient sorting capability allows separation of a 96-well plate within 1 min with a monoclonal rate of up to 90%. High-yielding cell lines were selected using a Naomcell single cell separator and stained directly at room temperature without the need for centrifugation to quickly pick up high-yielding single cells.