Determination of content According to high performance liquid chromatography (Appendix VID) determination. Chromatographic conditions and system suitability test The octadecylsilane bonded silica gel was used as the filler; methanol-acetic acid-water (35:4:65) was used as the mobile phase; the detection wavelength was 283 nm. The number of theoretical plates should not be less than 3,000 as calculated based on narinin peak. The control solution was accurately weighed and weighed at 110°C and dried to constant weight. The naringin reference substance was 20 mg. It was placed in a 100 ml volumetric flask. Methanol was added to the mark and shaken. The precise amount was 3 ml, and the 10 ml volumetric flask was filled with methanol. To the mark, shake, that is, (n = 60μg of naringin in each 1ml). Preparation of the test solution to take this product coarse powder about 0.25g (at the same time another take this product powder determination of moisture (Appendix IXH first method)), accurately weighed, add methanol 30ml, heat reflux for 3 hours, let cool, filter The filtrate was placed in a 50-ml volumetric flask, and the container was washed with a small amount of methanol several times. The washing solution was filtered into the same volumetric flask, and the methanol was added to the scale and shaken. The assay accurately draws 10 μl of each of the reference solution and the test solution, and is injected into the liquid chromatograph. This product is based on dry products, containing naringin (C27H32O14) not less than 0.50%.
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