Breeding mice with daily frozen testicular tissue can become a new method for preserving fertility

Breeding mice with daily frozen testicular tissue can become a new method for preserving fertility

Release date: 2014-07-03

In the July 1st issue of the British journal Nature News, a medical research paper reported that Japanese scientists used cryopreserved testicular tissue for the first time to produce live mouse offspring. This result indicates that cryopreservation of testicular tissue may be a realistic and important measure to preserve fertility.

According to the paper, infertility can be one of the side effects of certain cancer treatments. With the increase in the cure rate of childhood cancer, preserving fertility has become a concern of patients and their families. Since semen cryopreservation is only suitable for patients after puberty, younger patients need other alternatives.

The medical profession has been discussing for many years the possibility of retaining immature testicular tissue in accordance with the cryopreservation procedure and allowing the frozen tissue to resume the spermatogenic process. In the previous experimental observation, the testicular tissue of newborn mice was transplanted after cryopreservation for a period of time, and its performance was the same as that of fresh testicular tissue transplantation. The immature spermatogenic cells could continue to grow and develop in the recipient, and completed the whole spermatogenesis process. Then develop into sperm. But scientists have not yet developed live experimental mouse offspring.

Yokohama Yoshihiko, medical department of Yokohama City University, Japan, and his research team have previously developed an organ culture system that can induce the complete process of sperm production from the testes in mice. In the latest study, they use slow freezing or vitrification (a simple, rapid, and effective method of preserving living cells, tissues, and organs in frozen biology, in which the cell structure is not destroyed and the cells are Survival), cryopreservation of testicular tissue of newborn mice. These tissues were cultured after thawing. Studies have shown that the ability of these tissues to differentiate into sperm is as effective as the frozen tissue in the control group.

The team researchers then microinseminated the immature egg cells and placed them directly into the sperm. These spermatozoa were obtained from ultra-low temperature preservation of testicular tissue for 4 months, resulting in a total of 8 offspring. These offspring can grow healthily and can breed.

The results of this study provide a potential way to preserve fertility, providing a reliable and reliable experimental basis for saving male germ cells, helping cancer patients preserve their reproductive capacity, and preserving endangered species. But the team members also confessed that more research is needed to transform the results into humans.

Source: Technology Daily

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