John Dory Fish,Frozen Fish Fillets,John Dory Fish Fillet,Cream Dory Fish Fillet Skin Zhejiang Zhoushan Jiaze Aquatic Co., Ltd. , https://www.tianjia-aquatics.com
Pyrosequencing is a novel enzyme-linked sequencing technology developed by Nyren et al. in 1987. Pyrosequencing is suitable for sequencing of known short sequences, and its reproducibility and Accurate performance is comparable to Sanger DNA sequencing, and speed is greatly improved. Pyrosequencing technology products have the ability to simultaneously sequence and analyze a large number of samples, real-time quantitative analysis of single/continuous multiple nucleotide variants such as DNA methylation and SNP, which are large-throughput, low-cost, timely, rapid and intuitive. Detection provides an ideal platform for technical operation.
1. Principle of pyrosequencing technology Pyrosequencing technology is an enzyme cascade chemiluminescence reaction in the same reaction system catalyzed by four enzymes. The principle of pyrosequencing technology is: after the primer and template DNA are annealed, in DNA polymerase (DNA polymerase), ATP sulfurylase (ATP sulfurytase). Synergistic action of luciferase and apyrase, the polymerization of each dNTP on the primer is coupled with the release of a fluorescent signal, and the fluorescence release and intensity are detected to achieve real-time. The purpose of determining the DNA sequence. The reaction system of pyrosequencing technology consists of a reaction substrate, a single strand to be tested, a sequencing primer, and four enzymes. The reaction substrate was 5'-phosphosulfate (APS) and luciferin. 
2. Sulfation enzyme converts PPi to ATP, which causes luciferase to fluoresce (the intensity of light produced is proportional to the amount of bound nucleoside).
3. The excess dNTP is degraded and begins a new cycle. 
4. Sequencing results 
QIAGEN PyroMark Q24, PyroMark Q48 Autoprep, PyroMark Q96 ID Pyrosequencing 
Pyrosequencing experimental procedure 
Pyrosequencing technology application 
Whole genome methylation level detection: LUMA method 
For technical inquiries, please contact Shanghai Hipiji Biotechnology Co., Ltd. 021-54480369
Principle and application of pyrosequencing technology
Pyrosequencing technology
1. Each time a dNTP is added, a pyrophosphate (PPi) is produced by the polymerase;