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Huaan Bio's literature rewards activities have been going on for several years. I believe that many small partners have used our antibodies to publish articles and receive our literature rewards bonuses. In addition, our literature awards will be opened soon. Please pay more attention to it. If you have not submitted a reward application, please contact us.
Recently, we have received many literature award applications from small partners. Two of them have attracted the attention of Xiaobian. The contents of these two articles are related to zebrafish research. We all know that zebrafish is a common model organism, but there are very few antibodies against zebrafish in the market. We not only have more than one hundred zebrafish antibodies, but also can be customized according to customer needs. Let's take a look at these two articles.
01
Title : Sas10 controls ribosome biogenesis by stabilizing Mpp10 and delivering the Mpp10–Imp3–Imp4 complex to nucleolus
Author : College of Animal Science, Zhejiang University
Citation : Bhmt/ Sas10/ Mpp10/ Def/ (customized antibody) β-Actin (R1207-1) / C-Myc (0912-2)
PubMed ID : 30773582
Published magazine : Nucleic Acids Research
IF : 11.561
Antibody application : WB/IF 
Introduction to the introduction:
In eukaryotes, ribosomal biogenesis consumes more than 60% of the total energy of the cell, and the process involves transcription of pre-ribosomal RNA (rRNA); translation of mature ribosomal and non-ribosomal proteins for rRNA; Maturity of 18S, 5.8S and 28S rRNA and assembly of large and small ribosomal subunits. The smear small subunit (SSU) contains 18S rRNA and more than 30 ribosomal proteins. The biogenesis of the ribosomal small subunit originates from the processing and maturation of the 18S rRNA of the 35S (in yeast) pre-rRNA transcript, which is precisely controlled and stepwise. The process involves the involvement of approximately 70 non-ribosomal factors and various small nucleolar RNA (snoRNA). After transcription of the 5'-external transcribed spacer (5'-ETS) of 35S pre-rRNA, 5'-ETS recruits UTP-A protein and UTP-B protein complex, and then forms Mpp10 (mitotic phosphorylated protein 10, mitotic phosphate) Protein 10), Mpp10 interacting protein 3 (Imp3) and Mpp10 interacting protein 4 (Imp4) (ie, Mpp10-Imp3-Imp4 complex) and U3 small nucleolar ribonucleoprotein particle (snoRNP) complex). These complexes are assembled into a large complex called the 90S pre-ribosomes or SSU processed bodies. SSU processed bodies mediate 18S rRNA maturation by cleavage at the A0, A1 and A2 sites.
Summary:
Mpp10 forms a complex with Imp3 and Imp4 as a core component of the ribosome small subunit (SSU) processing group. Mpp10 also interacts with the nucleolar protein Sas10 / Utp3. However, it is still unknown how the Mpp10-Imp3-Imp4 complex is transferred to the nucleolus and the biological function of the Mpp10-Sas10 complex. The research team found that zebrafish Mpp10 and Sas10 are conserved nucleolar proteins, which are essential for the development of digestive organs. Mpp10, but not Sas10 / Utp3, is a target for the degradation pathway of the nucleolar-localized Def-Capn3 protein. Sas10 protects Mpp10 from Capn3-mediated cleavage by masking the Capn3 recognition site on Mpp10. Def interacts with Sas10 to form the Def-Sas10-Mpp10 complex to facilitate Capn3-mediated Mpp10 cleavage. Importantly, the authors found that Sas10 determines the nucleolar localization of the Mpp10-Imp3-Imp4 complex. In conclusion, Sas10 is not only essential for delivery of the Mpp10-Imp3-Imp4 complex to the nucleolus to assemble the SSU treatment group and to fine-tune the Mpp10 conversion in the nucleolus during organogenesis. 
Zebrafish Sas10 and Mpp10 are nucleolar proteins and display dynamic expression patterns during early embryogenesis. . (C) Western blot of Sas10, Mpp10, Def, Fib, Bhmt and α-Tub in total protein (Total), cytoplasmic fraction (CP) , nucleoplasmic fraction (NP) and nucleolar fraction (NO) from adult livers. (D–F) Co-immunostaining of Sas10 and Fib (D), Mpp10 and Fib (E), or Mpp10 and Sas10 (F) in the adult liver Big box shows the high magnification view of the nucleus in the small box. Nuclei were stained with DAPI; bar, 50 um.
02
Title : Aloe emodin induces hepatotoxicity by activating NF-κB inflammatory pathway and P53 apoptosis pathway in zebrafish
Author unit : Chengdu University of Traditional Chinese Medicine
Reference antibodies : P65 (ET1603-12), P53 (ET1601-13), JNK (ET1601-28), GAPDH (M1310-2)
PubMed ID : 30771440
Published magazine : Toxicology Letters
IF : 3.166
Antibody application : WB 
Introduction to the introduction:
AE (Aloe emodin, aloe emodin) is present in many natural products with a wide range of pharmacological activities, and AE has been reported to have hepatotoxic effects. In this study, the researchers studied the hepatotoxic mechanisms of AE primarily from the inflammatory and apoptotic pathways. Molecular docking was first used to explore the affinity between the AE and the two pathways, and then the zebrafish was selected as an animal model to verify the reliability of molecular docking results in vivo. 
Summary:
The purpose of this study was to investigate the hepatotoxic effects of aloe-emodin (AE) and its underlying mechanisms. Using molecular docking technology, AE docked with the NF-κB inflammatory pathway and the P53 apoptotic pathway target, respectively. The results of molecular docking were verified and the hepatotoxic mechanism of AE was further investigated. Zebrafish Tg (fabp10: EGFP) was used as an in vivo animal model. The pathological sections of the zebrafish liver were analyzed to observe histopathological changes, and Sudan Black B was used to study whether there was an inflammatory reaction in the zebrafish liver. Then TdT-mediated dUTP Nick-End Labeling (TUNEL) was used to detect apoptosis signals in zebrafish hepatocytes. Finally, qRT-PCR and Western Blot were used to measure mRNA and target protein expression in zebrafish NF-κB and P53 pathways, respectively. Level. Molecular docking results indicate that AE can successfully interface with all targets of the NF-κB and P53 pathways, and most targets have a docking score equal to or higher than the corresponding ligand. Pathological sections showed that AE caused zebrafish liver lesions, and Sudan black B staining showed that AE caused Sudan black B to stain zebrafish liver. TUNEL assay then showed a large number of dense apoptotic signals in the AE group, mainly distributed in the liver and yolk sac of zebrafish. qRT-PCR and Western blot results showed that AE increased the mRNA and protein expression levels of pro-inflammatory and pro-apoptotic targets in the NF-κB and P53 pathways. AE can activate the NF-κB inflammatory pathway and the P53 apoptotic pathway, and its hepatotoxic mechanism is related to the activation of NF-κB-P53 in the inflammatory-apoptotic pathway. 
If you need antibodies, you can contact us. You can choose the antibodies in the catalog. We can also customize the products for you without antibodies. In addition, we are about to participate in the International Zebrafish Conference held in Suzhou in June 2019. Interested children's shoes can be contacted at the show!
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2019 "Hua'an Cup - Dandelion Award" high score literature sharing - zebrafish basic research articles
Huaan antibody helps scientists renew new literature in zebrafish basic research
Fig. The protein expression level of the key protein in NF-κB and P53 pathways.