Comparison of Kinetics and Affinity Detection Results of the Same Group of Proteins and Antibodies by OpenSPR, Biacore and ForteBio

Leading the past, see today's OpenSPR, Biacore and ForteBio are weak and weak?

In modern biology, medicine and translational medicine, pharmacology and other research, with the deepening of functional genomics research, the biological function research of biological size molecules occupies a very important position. Molecular interaction analyzer has become a research in the field of life science. An indispensable important tool, the most classic of which is GE's Biacore SPR technology. In recent years, many new brands have been developed on the market. As for the cowardice, it is difficult to justify it. Only the facts speak louder than words. Show you the results of the same group of proteins and antibodies detected by the mainstream OpenSPR, Biacore and ForteBio on the market:

Experimental content
1. Comparison of the binding kinetics of biotinylated CD16a and IgG using OpenSPRTM, BiacoreTM and OctetTM;
2. The KD values ​​measured by OpenSPRTM, BiacoreTM and OctetTM are 74.7 nM, 48.7 nM and 226 nM, respectively;
3. This study demonstrates that OpenSPR is equivalent to other commercial instruments used to analyze binding kinetics and affinities;

OpenSPRTM is a powerful instrument that provides in-depth, label-free binding kinetics detection and analysis for a wide variety of molecular interactions. To demonstrate the power and accuracy of OpenSPRTM, we compared the BiacoreTM 3000 and OctetTMRED96 test data. BiacoreTM and OctetTM systems are commonly used to analyze binding kinetics, but cost hundreds of thousands of dollars, so many researchers who need them can't use them. To demonstrate that OpenSPRTM can achieve comparable results to BiacoreTM and OctetTM at a lower cost, we used these three instruments to analyze Fc-FcR interactions under similar conditions.

What is the cost of OpenSPRTM compared to the industry standard BiacoreTM or OctetTM?

OpenSPR is a desktop SPR solution that provides researchers with the ability to perform in-depth, label-free binding kinetics testing in their own labs. Compared to the standard SPR or BLI solutions in the pharmaceutical industry, BiacoreTM SPR and OctetTM BLI instruments cost hundreds of thousands of dollars, while OpenSPR requires almost half the price, so it can be obtained at a fraction of the cost. BiacoreTM and OctetTM have the same data, and the instrument delivers fast results and simple operation, allowing researchers to research and publish research faster than BiacoreTM and OctetTM systems.

Comparative study: Interaction analysis of Fc-FcR by OpenSPRTM, BiacoreTM and OctetTM

In the study, we examined the Fc-FcR interaction binding kinetics using OpenSPRTM, BiacoreTM 3000 and OctetTM RED96. BiacoreTM and OctetTM experiments were performed by CRO trained technicians, and OpenSPR experiments were performed by Nicoya scientists. The purpose of this study was to verify the equivalence of OpenSPR with other commercial instruments used to analyze binding kinetics and affinities. The kon, koff and KD of the Fc-FcR interaction were analyzed by kinetic analysis. The specific results are as follows ( Note: If you want to know more about the materials, protocols and equipment used in this comparison, you can check our detailed technology. Description ):

result

The Fc-FcR interaction results detected with OpenSPRTM, BiacoreTM and OctetTM instruments are shown in Figures 1, 2, 3 and Table 1, respectively. Each experimental data was fitted using a 1:1 binding model. The KD values ​​determined by OpenSPRTM, BiacoreTM and OctetTM were 74.7 nM, 48.7 nM and 226 nM, respectively. The calculated KD values ​​between OpenSPR and BiacoreTM 3000 are very close, while the values ​​reported by OctetTM differ by about 5 times. The kinetic constants obtained by fitting are shown in Table 1. The dissociation rates of the three instruments are similar, but the difference in the on-rates of OctetTM is over an order of magnitude. The OpenSPRTM and BiacoreTM fits are of comparable quality, but the OctetTM data has a large deviation.

Due to the characteristics and operation limitations of the instrument itself, even when repeated experiments are performed on the same instrument, the measured changes in affinity and kinetic values ​​are often seen. The closeness of the experimental values ​​of BiacoreTM and OpenSPRTM demonstrates the comparability of these instruments for biomolecular interaction analysis. The small differences observed may be due to small differences in precise experimental conditions and materials used. Large differences in Octet data may be due to the use of higher fixed levels, fitting differences, or basic differences in dip and read techniques used by BLI.

in conclusion

In summary, OpenSPRTM and BiacoreTM instruments achieve similar kinetic and affinity values ​​for the same set of experiments, strongly supporting the detection and study of accurate and economical intermolecular binding interactions using OpenSPRTM.