Customer Literature | The first non-target metabolomics study of sera patients with syphilis was published!

Syphilis is a common sexually transmitted disease worldwide. The pathogen is treponema pallidum. In 2017, China CDC reported 475,860 new cases of syphilis and 45 deaths, ranking third among all Class B infectious diseases. Bit.

Today, Xiaobian wants to interpret a recent article published by Yang Tianci, a group of APT clients from Zhongshan University Hospital of Xiamen University. It is the first article on metabolomics research in patients with syphilis and has screened a marker that can be used for the diagnosis of syphilis. Objects, in which Zhongkexin Life provides non-targeted metabolomics testing.

The diagnosis and pathogenesis of syphilis remains a difficult point in clinical research. At present, the diagnosis of syphilis mainly depends on serological tests (including non-treplidose and Treponema antibody detection). The false negative results of serological tests and the limitations of this method may hinder the timely diagnosis and treatment of syphilis. Scientists are still looking for more sensitive. More specific syphilis diagnostic markers.

In this study, non-target metabolomics was used for the first time to search for metabolic markers in serum of patients with syphilis.

Analysis of Serum Metabolite Profiles in Syphilis Patients by Untargeted Metabolomics

2019 IF=4.287

Original link: https://onlinelibrary.wiley.com/doi/10.1111/jdv.15530

Study materials: serum samples, 20 patients with syphilis, 20 healthy people

Research result:

01, quality control evaluation

The individualized differences in metabolism are large, the number of biological repeats is large, and no mixing is performed, so the requirements for stability of the instrument are high. Through the QC sample quality control evaluation, the stability of the instrument can be evaluated to judge the quality of the data.

Through PCA analysis, it can be seen that all the QC samples (blue dots) in the figure are closely clustered together, indicating that the instrument has good stability and reliable data during the experiment.

02, serum metabolomics results

Based on UPLC-Q-TOF/MS technology, 2890 characteristic peaks were identified. PLS-DA and hierarchical clustering heat map analysis showed that syphilis patients and healthy groups had obvious separation tendency at metabolic level.

03, screening potential biomarkers

There are five significant metabolites that meet the VIP>1, P<0.05 differential screening criteria, namely, oxide trimethylamine, arginine, hemolytic phosphatidylcholine, betaine, and acetylcarnitine. Among them, the trimethylamine oxide in patients with syphilis is 3.922 times that of the healthy control group, which can be used as a metabolic diagnostic marker for patients with syphilis and healthy people.

04. Path analysis

KEGG pathway analysis indicated that metabolites are mainly involved in glycerophospholipid metabolism, choline metabolism, and ABC transporters.

05, the results

This study, for the first time, through non-target metabolomics , revealed a significant difference in the metabolic level between syphilis patients and healthy individuals. Oxide trimethylamine can be used as a diagnostic marker for patients with syphilis.

Xiaobian experience

Novel research samples and directions may be one of the reasons why this article was published in the JCR 2 area. On the one hand, the authors can use a large sample cohort to verify the diagnostic ability of oxide trimethylamine. On the other hand, it can also conduct a more in-depth mechanism study from the abnormal metabolic pathway, combined with the proteome and transcriptome.

Zhongke New Life launched the metabolomics service as early as 2014. It is one of the earliest platforms to provide metabolomics services on the market. 2700+ metabolite standard secondary spectrum database + manual secondary confirmation, double guarantee qualitative results Welcome teachers with testing needs to come to consult.

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