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1 Materials and Methods
1.1 Broodstock Breeding and Selection
1.1.1 Broodstock Breeding Broodstock is cultivated with a special pond. The pool area is 1,000 square meters and the water depth is about 1.5 meters. There are small bottom fish such as squid and quail in the pool, and they are regularly fed about 7 centimeters of quail and squid. Species. The water is flushed every 10 to 15 days, and flushed every 2 to 3 days before the urine production.
In order to ensure sufficient oxygen in the brooding pond, an aerator was installed in the pool.
1.1.2 Select broodstock to choose a healthy, non-injured pro-pigs, males are generally 2 instars, and females are generally older than 3 years old. In mature males, the appearance of gonads was punctiformly small, and milky white semen flowed out from the abdomen and the water was scattered. The gonads of mature females showed a "one" shape, the abdomen expanded, the outline of the ovary was clear, the genital holes were loose, the abdomen was soft pressed, and the abdomen was soft and elastic. The ventral midline was concave.
1.2 Aphrodisiac, spawning and hatching
1.2.1 HCG+LRH-A was used as an aphrodisiac aphrodisiac, and a single injection into the thoracic cavity was used. The dose was HCG100 unit 10 LRH-A50 μg/kg, and 3 batches of oxytocin were produced on June 7, 1999, June 9, and June 15, 2000, respectively. get on. The aphrodisiac situation is shown in Table 1.
1.2.2 Spawning Injecting aphrodisiac broodstock into the spawning pool in order to prevent the broodstock from consuming too much energy and flushing water after 16 hours of standing water to promote broodstock oviposition. Water temperature 24 - 27 °C effect time is 23 - 24 hours, when the egg in the collection box appears, you can prepare for eggs.
1.2.3 Incubation The fertilized eggs are put into the loop for running water incubation. Because the eggs of the carp are semi-buoyant eggs with no stickiness, and they have oil balls, which are small in size and large in specific gravity, they are easy to sink. Correspondingly larger, it is better to do not sink eggs. Incubation water must be filtered with 60 mesh nylon mesh. During the incubation process, the embryonic development of fish eggs was constantly observed. The fertility rate was checked in the middle of the gastrointestine and the hatching rate was calculated after the film was released. Water temperature 24 - 27 °C, about 32 hours after the rupture of membrane emergence. The newly hatched larvae have a total length of 3.2–4.1 mm and a large yolk sac. After 3 days of filming, the larvae have the ability to balance and can swim flatly. Some fish fry open their food; after 4 days, they can all feed.
1.3 After larvae have been cultivated, the larvae will continue to be cultivated in the ring. The carp larvae prefer to have small but long-lived fishes with relatively weak swimming ability. This experiment used carp seedlings as the opening bait for squid, and the larvae were before the opening. On the 1st day, the "fish nest" of the head of the bolete head hanging in the membrane was hung in the ring road. In the first 3 days after the opening, a large amount of head lice and grass carp were continuously fed. After the opening, they were fed for 4 to 6 days. The eel and eel splashes are transferred to the pond for cultivation after the 6th day. The ponds are fed with sufficient numbers of grass carp and carp seedlings every day. With the growth of the carp seedlings, the size of the feed fish is also correspondingly increased. After 15 to 20 days of cultivation, a fry of 2 to 3 cm in length can be cultivated. The requirements for water quality are high for carp. During the cultivation of the seedlings, the water in the pool must be kept to a sufficient level of dissolved oxygen, new water must be continuously added to the roundabout, and the ring filter should be washed every day; the ponds should also be frequently raised. Water to keep the water fresh. Fish disease should be promptly treated.
2 Test Results and Discussion
2.1 Results 1999 - a total of 3 batches of oxytocin were produced in 38 years, totaling 38 groups, with a total of 1.11 million spawning. Three batches of squid had emerged with 180,000, 200,000 and 250,000 respectively; the hatching rates were 66% and 59%, respectively. , 70%; Breed 2 to 3 cm of fish respectively 61,000, 75,000, 100,000, survival rate were 34%, 38%, 40%.
2.2 Discussion
2.2.1 Breeding broodstock is the key to the artificial propagation of squid. In mid-March, brood broodstock should be intensively cultivated in special ponds, and be fed with a sufficient number of pupae and squid for bait fish every few days. Regular flushing should be carried out to increase water flow stimulation and accelerate gonad development in broodstock.
2.2.2 In Shandong, artificial oxytocin production can be performed when the water temperature stabilizes above 20°C in late May. Under possible conditions, oxytocin should be advanced as early as possible, and it is best to synchronize production with the oyster production to ensure that there are sufficient bait fish.
2.2.3 The key to improving the survival rate of carp seedlings is the opening bait. The carp's opening bait is preferably fresh carp and grass carp seedlings. The start of the baiting of the bait fish is started on the 2nd day after the catfish rupture.
2.2.4 Seed breeding should be done by selecting cement ponds with small water bodies, incubation loops or cages to keep the water fresh, with sufficient dissolved oxygen, and preferably with micro-flowing water. Daily feeding of bait-rich fish is preferable.
2.2.5 The water used for hatching should be filtered to prevent the entry of sword blisters. Otherwise it will seriously damage the fish that has been stripped or has gone out of membrane.
Artificial breeding and seed breeding of carp
Siniperca chuatsi, also known as seasonal fish, is a valuable fish in China. Its delicious meat, no intermuscular puncture, strong disease resistance, easy breeding, and high selling price make it a treasure in high-end banquets. In recent years, small-scale tests have been carried out in some areas of our province and have achieved certain effects, but due to the constraints of provenance and aquaculture technology, development has been slow. In order to meet the need for large-scale propagation of seedlings for breeding eel, the author conducted a study on the artificial propagation and seed-cultivation techniques of carp from 1999 to 2000. Now the research results for the past two years are reported below.
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