I. Brief Introduction: The Neutral Protease is a solid enzyme produced by submerged fermentation from Bacillus Subtilis according to the specification of GB/T 23527-2009 (China). It is widely applied in detergent, leather depilation and softening, silk degumming, toothpaste, soap, baking, brewing industry, dairy product, animal feed, medicine industry and etc. II. Definition of Activity: One gram of solid Neutral Protease powder at 30°C and pH 7.5 hydrolyze casein in one minute to produce 1μg tyrosine is defined as one enzyme activity unit and marked with u/g. III. Characteristics: 1. Neutral Protease can efficiently hydrolyze the protein into peptone and peptides and finally into free amino acids. 2. Optimum temperature: 40-50°C Optimum pH: 6.5-7.5 IV. Specifications: V. Storage: Should be stored in a cool, dry and well-ventilated place; protect from direct sunlight, heat and dampness; avoid storing with poisonous and harmful substances. VI. Shelf Life: Enzyme activity remains ≥ 90% after 12 months at room temperature 25°C. VII. Packaging: 20kg/bag, 25kg/bag or as clients' requirements IX. Precautions: Enzyme preparations belong to protein, which may induce sensitization and cause allergic type reactions in sensitized individuals. Prolonged contact may cause minor irritation for skin, eyes or mucous membrane of nose, so any direct contiguity with human body should be avoided. If irritation or allergic response for skin or eyes develops, please consult a doctor. I. Brief Introduction: The Neutral Protease is a solid enzyme produced by submerged fermentation from Bacillus Subtilis according to the specification of GB/T 23527-2009 (China). It is widely applied in detergent, leather depilation and softening, silk degumming, toothpaste, soap, baking, brewing industry, dairy product, animal feed, medicine industry and etc. II. Definition of Activity: One gram of solid Neutral Protease powder at 30°C and pH 7.5 hydrolyze casein in one minute to produce 1μg tyrosine is defined as one enzyme activity unit and marked with u/g. III. Characteristics: 1. Neutral Protease can efficiently hydrolyze the protein into peptone and peptides and finally into free amino acids. 2. Optimum temperature: 40-50°C Optimum pH: 6.5-7.5 IV. Specifications: V. Storage: Should be stored in a cool, dry and well-ventilated place; protect from direct sunlight, heat and dampness; avoid storing with poisonous and harmful substances. VI. Shelf Life: Enzyme activity remains ≥ 90% after 12 months at room temperature 25°C. VII. Packaging: 20kg/bag, 25kg/bag or as clients' requirements IX. Precautions: Enzyme preparations belong to protein, which may induce sensitization and cause allergic type reactions in sensitized individuals. Prolonged contact may cause minor irritation for skin, eyes or mucous membrane of nose, so any direct contiguity with human body should be avoided. If irritation or allergic response for skin or eyes develops, please consult a doctor.
Plant extraction process
1. Select plants/medicinal materials. It is nothing more than ancient prescriptions, proven prescriptions, and folk medicinal herbs. At present, common and uncommon medicinal materials have been studied. Most of the time, the amount of medicinal materials has been increased to extract low-isolated components, or medicinal plants have not been studied from Miao medicine, Tibetan medicine, Mongolian medicine, Africa, Latin America and other places.
2. Extract. Solvent petroleum ether, n-hexane, cyclohexane, benzene, chloroform, ethyl acetate, n-butanol, acetone, ethanol, methanol, water (small polarity → large polarity). Daily decoction of medicines is effective, use water and ethanol and other solvents with high polarity. Artemisinin and other boiling methods are not effective, use petroleum ether and other solvents with low polarity. The common medicinal materials, water/alcohol/ether, are presented again, and more compounds can be separated and identified.
3. Separation. This is the most important task. There are dozens of compounds in the solution extracted in the second step. Generally, column chromatography is used, which is what we often call column flushing. The workload is large, boring, and low-tech. A master's degree may do this every day for 2 years of experimentation. As shown in the figure below, the column for separating compounds is as large as 2 meters high and as small as 10 cm. Change the solvent conditions of the mobile phase, change the material of the column, and repeatedly wash the column under different conditions and separation principles to separate the monomer compound. High Content Monomer,Kudzu Root Powder,Magnolia Bark Extract Powder,Golden Turtle Lotus Extract Shaanxi HuiKe Botanical Development Co.,Ltd , https://www.oasis-hk.com
Appearance
yellow-brown powder
Odor
slight fermentation odor
Enzyme Activity
50,000u/g, 100,000u/g
Water Content
≤ 8%
Particle Size
≥ 80% pass 40 mesh sieve
About Us
Yonkong Bioengineering Co.,Ltd.(Chinese Name: Hangzhou Yanghong Bioengineering Co.,Ltd.) has been specializing in the field of enzymes specialized in enzymes and their applications.The products line started from feed enzymes and gradually comes to brewing enzymes,paper making enzymes,textile enzymes, baking enzymes and so on. Main products- phytase, xylanase, mannanase, alpha-galactosidase,cellulase, beta-glucanase, amylase, protease and complex enzymes have already gained great honor in United States, Russia, Ukraine,Thailand, Vietnam, South Korea and other regions.Â
Appearance
yellow-brown powder
Odor
slight fermentation odor
Enzyme Activity
50,000u/g, 100,000u/g
Water Content
≤ 8%
Particle Size
≥ 80% pass 40 mesh sieve
About Us
Yonkong Bioengineering Co.,Ltd.(Chinese Name: Hangzhou Yanghong Bioengineering Co.,Ltd.) has been specializing in the field of enzymes specialized in enzymes and their applications.The products line started from feed enzymes and gradually comes to brewing enzymes,paper making enzymes,textile enzymes, baking enzymes and so on. Main products- phytase, xylanase, mannanase, alpha-galactosidase,cellulase, beta-glucanase, amylase, protease and complex enzymes have already gained great honor in United States, Russia, Ukraine,Thailand, Vietnam, South Korea and other regions.Â
Hot Sale Neutral Protease for Animal Feed, Brewing Medicine Industry>
Model NO.: Neutral Protease, Protease Enzyme, Protease
Specification: 20kg/bag, 25kg/bag
Origin: Hebei, China (Mainland)
HS Code: 3507909000
Model NO.: Neutral Protease, Protease Enzyme, Protease
Specification: 20kg/bag, 25kg/bag
Origin: Hebei, China (Mainland)
HS Code: 3507909000