Ganoderma lucidum cultivation new technology

Ganoderma lucidum cultivation new technology

Ganoderma lucidum is a traditional Chinese fungal drug. As a traditional Chinese medicine and longevity supplement, it has a history of a thousand years. The domestic market has great potential, and the development of Ganoderma lucidum production industry has broad prospects. Now introduce Ganoderma lucidum short section wood cultivation technology methods: First, cultivation of raw and auxiliary materials cultivation of Ganoderma lucidum is a good tree species Fagaceae, Hamamelidaceae, birch families and other tree species. General section of the wood to choose a thick bark, difficult to break away, the material is hard, less heartwood, marrow ray developed, rich catheters, tree diameter of 8cm ~ 13cm is appropriate? In the early fall of leaves, not more than convulsions. Second, the selection of cultivation season Ganoderma lucidum belongs to the high-temperature solid bacteria. 10 °C ~ 12 °C for the cultivation of tube production period. Short-segmented wood must be cultured for 60-75 days before it reaches physiological maturity. Third, the establishment of cultivation venues The best choice for outdoor cultivation soil loose, open terrain, water, convenient places as a cultivation field. The cultivation field should cover 2m to 2.2m in height and 4m in width. The shed should be divided into two sections, with a width of 1.5m and a drainage ditch. If conditions permit, the roof can be covered with a black shade net, the shading rate is 65%, so that a strong scattered light is formed in the shed, and the service life is as long as 3 years or more. Fourth, the filler is used on the low-pressure polyethylene cylinder with a diameter of 15 to 24cm55cm and 0.02cm. Most of the three plastic barrels are used in production so that they can be used for short-section wood cultivation with different calibers. The short section of wood after the interception is put into a plastic tube, the two ends are twisted together, bent, and the head is tied with a small rope and fastened. The use of plastic barrels with a diameter of 2 to 3 cm larger than the section wood, 30 cm long sections of wood, one section of each bag, 15 cm sections of wood, one section of two bags, and several sections can be bundled together into one bag to be sterilized. 5. Sterilization followed immediately by normal atmospheric pressure sterilization at 97°C to 103°C for 10 to 12 hours. Six, inoculation selection Marketable, good quality, high yield varieties for the production of strains, the current strains used g801, g802, g6, g8 and so on. The preparation method is the same as that of the wood rot fungi, and the use of sawdust, cottonseed shells, and the like are better. When the wood is inoculated, the water content of the strain is slightly better. The cooled short plastic wooden barrel is pre-selected and sterilized with an aerosol disinfection box. Thirty minutes later, the skin of the plastic bag was discarded and the double-headed inoculation method was used. With the help of the two, one person untied the plastic tying rope, and the other sputtered the bred peanut-sized bacteria near the mouth of the alcohol flame, and immediately sealed it tightly. The other end is vaccinated the same way, and so on, and then stacked on top of the shelves. The inoculation process should shorten the opening time as much as possible, increase the amount of inoculum, seal the section, reduce pollution, and quickly spread the mycelium along the wood beam of the short section of wood. VII. To cultivate winters with lower temperatures, artificial warming should be applied to temperatures above 20°C. After 15 to 20 days of cultivation, loosen the ropes slightly. The short section of wood culture 45 ~ 55 days full tube, full tube after another 15 to 20 days before entering the physiological maturity phase, this time can only be down. Eight, the parade will be buried in the short-skinned mature wood lateral surface, paragraph wood lateral spacing of 3cm. This method of horizontal burying works better than vertical burial. The final full cover soil, the thickness of 2 ~ 3cm. Watering heavily for two consecutive days. Every 200cm, short arches were erected with bamboo pieces, 15cm above the ground, covered with film, and both ends slightly open. The soil moisture in the buried soil is 20% to 22%, and the air relative humidity is about 90%. Nine, the development of Zizhi fruiting body development temperature of 22 °C ~ 35 °C, into the lotus root to keep the surface wet, with the finger squeezed soil particles have a crack for the degree, rather partial dry. In the middle and late May, the jujube breaks through the ground and the water management is dominated by alternating wet and dry. At night, it is necessary to close the membrane on both sides of the shed to allow it to be moisturized and then opened again during the day to prevent the surface of carbon dioxide from being excessively high, exceeding 0.1%, resulting in "antler antler", without disintegrating the cap of the bacterium, and only having a long handle. Ventilation is the key to guarantee the normal development of Ganoderma lucidum cap. After June, the top film of the shed must always be covered, and both sides should be opened to prevent rain and soil from causing high humidity in the soil and wood. In mid-to late June, thicker shades are often used to ensure a higher relative humidity in the air. When the surface exhibits a lacquer-like luster, spores or fruiting bodies can be collected. X. Harvesting and Drying When the cap is no longer enlarged, the white edge disappears, the cover edge has multiple layers of thickening, the handle cover has the same color, and the spores can be collected when they are scattered. The harvested fruit body should be cut off with the stipe of the sediment, baked at 40 °C ~ 60 °C to a moisture content of less than 12%, and finally sealed and stored in plastic bags. Pleurotus ostreatus increase "artificial stimulation" ten methods of temperature difference stimulation in the formation phase of Pleurotus ostreatus fruit body, given a daily temperature difference of 7 °C ~ 12 °C stimulation, can promote its early mushrooming. The method is: cover film insulation during the day, sunny morning and evening to expose the film exposed bed, increase the temperature difference through cooling, and combined with high temperature watering induced mushroom. The high-temperature stimulation method first dried the fungus bed (or bacterial bag) for 1 to 2 days, and then continuously re-spray the water, so that a large amount of water accumulated on the bacteria surface, so that the bacteria bed (or bacteria) slowly absorbed, spray water every day 2 to 3 times for 2 to 3 consecutive days. Finally, the water on the surface of the bacteria is sucked off with a cotton cloth, covered with a plastic film, and it can be budded after a few days. After the hyphae stimulation method is used to fill the hyphae in the culture medium, it is squeezed, beaten, and shaken with the elastic wood strip to promote the accelerated growth of the hyphae and the rapid differentiation of the fruit body, generally increasing the yield by about 15%. However, the force is required to be uniform so as not to destroy the material surface. The pressure stimulating method covers the surface of the culture material with old newspapers, sacks, and fine soils that have been sterilized. Under the stimulation of pressure, the mycelium can be rapidly grown. The light-induced mushroom house is planted with oyster mushrooms. When the fruit body is formed, a certain amount of scattered light is required. Pleurotus ostreatus should be germinated in dark conditions after sowing, until the mycelium is well exposed and exposure can induce mushroom. In the absence of light, the lamp can be used instead, and it also has a good stimulating effect. Hormone stimulation in the young bud stage spraying with 0.5 ~ 1ppm triacontanol can increase production by 10% to 15%. In the period of mushroom bud, young mushroom, and the expansion of the cap, 500 ppm of ethylene can be sprayed three times, each time 50 ml per square meter, can increase production by 20%. Compounding agent stimulation method than the long-term (b9) 5g, add magnesium sulfate 20g, boric acid 5g, zinc sulfate 10g, vitamin b1250g, urea 50g, water 50kg, formulated as a compound liquid, sprayed in the mushroom bud formation period in the culture On the material surface, it can promote fruit body formation and hypertrophy. Ethanol-stimulating ethanol 2%, potassium dihydrogen phosphate 0.1%, and vitamin c 0.02% can increase production by more than 15% during spraying of mushroom buds, but should be used with caution when the weather is hot. Monosodium glutamate MSG 0.1%, glucose 1% plus vitamin b1100 tablets of water, spraying about 7 days after the first mushroom harvest, 0.5 to 1 kg per square meter of liquid, sharing 3 to 4 times , can increase production by 15% to 20%. Grass ash stimulation method Take 5% of the grass ash immersed in the clear solution for spraying, can promote the growth of mycelium, mushroom sturdiness, and to prevent the mycelium from turning red, usually sprayed after harvesting each batch of mushrooms, can increase production by about 20%.

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