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BMC Plant Biology: Second-generation sequencing to identify wild soybean miRNA and its target gene under aluminum stress
MicroRNAs (miRNAs) are a class of endogenous non-coding small RNAs that regulate a wide range of physiological processes in eukaryotes. Their regulation is achieved by degrading target mRNA or hindering its translation. Plant miRNAs are usually completely or nearly perfectly matched to their target genes, causing degradation of mRNA. In plants, miRNAs are involved in the regulation of developmental processes and responses to biotic or abiotic stresses. To determine the function of miRNAs, they must first identify their target genes. Degradation group sequencing is a recent and highly effective method for large-scale identification of plant miRNA target genes.
Professor Nian Hai's research group constructed two small RNA sequencing libraries and two degradation group sequencing libraries for the roots of wild soybean seedlings treated with aluminum and aluminum. 97 known miRNAs and 31 new miRNAs in wild soybeans were identified by miRNA sequencing. In addition, miRNAs on the 3P or 5P chain corresponding to 49 known miRNAs were also found. Of all identified miRNAs, expression of 30 miRNAs was associated with aluminum stress. Further using degradation group sequencing, the authors identified 86 target genes of known miRNAs and five target genes for sequencing to find miRNAs. The results of GO enrichment analysis showed that the target genes of 52 conserved miRNA families play an important role in transcriptional regulation. It was also found that some genes involved in stress response, such as auxin response factor (ARF), were cleaved by miRNA under aluminum stress conditions.
This study combined miRNA sequencing and degradation group sequencing technology to systematically determine the miRNA expression profile and target genes and functions of wild soybean under aluminum stress, which provides important information for understanding the regulation of miRNA under aluminum stress.
The small RNA sequencing and degradation group sequencing work in this study was provided by Lianchuan Biotech.
Lianchuan Biological Small RNA Sequencing and Degradation Group Sequencing Users Recently Published Article Index
l Li, M. et al. (2012) An atlas of DNA methylomes in porcine adipose and muscle tissues. Nat Commun 3, 850.
l Zhai L, Liu Z, Zou X, Jiang Y, Qiu F, Zheng and Y, Zhang Z. (2012) Genome-wide identification and analysis of microRNA responding to long-term waterlogging in crown roots of maize seedlings. Physiologia Plantarum [Epub Ahead of print].
l Zeng QY, Yang CY, Ma QB, Li XP, Dong WW, Nian H. (2012) Identification of wild soybean miRNAs and their target genes responsive to aluminum stress. BMC Plant Biol 12(1), 182.
l Xu MY, Dong Y, Zhang QX, Zhang L, Luo YZ, Sun J, Fan YL, Wang L. (2012) Identification of miRNAs and their targets from Brassica napus by high-throughput sequencing and degradome analysis. BMC Genomics 13: 421.
l Huang T, Xu D, Zhang X. (2012) Characterization of host microRNAs that respond to DNA virus infection in a crustacean. BMC Genomics 13(1), 159.
l Yang G, Yang L, Zhao Z, Wang J, Zhang X (2012) Signature miRNAs Involved in the Innate Immunity of Invertebrates. PLoS ONE 7(6), e39015.
l Li R, Sun Q, Jia Y, Cong R, Ni Y, et al. (2012) Coordinated miRNA/mRNA Expression Profiles for Understanding Breed-Specific Metabolic Characters of Liver between Erhualian and Large White Pigs. PLoS ONE 7(6) , e38716.
l Mao W, Li Z, Xia X, Li Y, Yu J. (2012) A Combined Approach of High-Throughput Sequencing and Degradome Analysis Reveals Tissue Specific Expression of MicroRNAs and Their Targets in Cucumber. PLoS One 7(3), e33040
l Zhao M, Tai H, Sun S, Zhang F, Xu Y, et al. (2012) Cloning and Characterization of Maize miRNAs Involved in Responses to Nitrogen Deficiency. PLoS ONE 7(1), e29669.
l Zhang J, Zhang S, Han S, Wu T, Li X, Li W, Qi L. (2012) Genome-wide identification of microRNAs in larch and stage-specific modulation of 11 conserved microRNAs and their targets during somatic embryogenesis. Planta [Epub ahead of print]
l Zhou Q, Li M, Wang X, Li Q, Wang T, Zhu Q, Zhou X, Wang X, Gao X, Li X. (2012) Immune-related MicroRNAs are Abundant in Breast Milk Exosomes. Int J Biol Sci 8 (1), 118-123.
l Li B, Qin Y, Duan H, Yin W, Xia X. (2011) Genome-wide characterization of new and drought stress responsive microRNAs in Populus euphratica. J Exp Bot 62(11), 3765-79.